Block Type 96-Well 0.1-mL Block
Calibration Background is expired No
Calibration Background performed on 09-20-2022
Calibration Pure Dye ABY is expired No
Calibration Pure Dye ABY performed on 09-20-2022
Calibration Pure Dye CY5 is expired No
Calibration Pure Dye CY5 performed on 09-20-2022
Calibration Pure Dye FAM is expired No
Calibration Pure Dye FAM performed on 09-20-2022
Calibration Pure Dye JUN is expired No
Calibration Pure Dye JUN performed on 09-20-2022
Calibration Pure Dye MUSTANG PURPLE is expired No
Calibration Pure Dye MUSTANG PURPLE performed on 09-20-2022
Calibration Pure Dye NED is expired No
Calibration Pure Dye NED performed on 09-20-2022
Calibration Pure Dye ROX is expired No
Calibration Pure Dye ROX performed on 09-20-2022
Calibration Pure Dye SYBR is expired No
Calibration Pure Dye SYBR performed on 09-20-2022
Calibration Pure Dye TAMRA is expired No
Calibration Pure Dye TAMRA performed on 09-20-2022
Calibration Pure Dye VIC is expired No
Calibration Pure Dye VIC performed on 09-20-2022
Calibration ROI is expired No
Calibration ROI performed on 09-20-2022
Calibration Uniformity is expired No
Calibration Uniformity performed on 09-20-2022
Chemistry SYBR_GREEN
Date Created 2022-12-22 09:06:36 AM SGT
Experiment Barcode NA
Experiment Comment NA
Experiment File Name D:BiosystemsDesign & Analysis SoftwareFiles\2022-12-21_155355.eds
Experiment Name 2022-12-21_155355
Experiment Run End Time 2022-12-21 17:41:38 PM SGT
Experiment Type Standard Curve
Instrument Name 272511079
Instrument Serial Number 272511079
Instrument Type QuantStudio™ 5 System
Passive Reference ROX
Post-read Stage/Step NA
Pre-read Stage/Step NA
Quantification Cycle Method Ct
Signal Smoothing On true
Stage where Melt Analysis is performed Stage3
Stage/ Cycle where Ct Analysis is performed Stage2, Step2
User Name Deborah Chen

Assay Controls:

Controls Summary
Negative Control Assay is invalidated see below for details.
Positive Control Assay is invalidated due to failed Positive control.
Inhibition Control Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.

1. Negative Control (NTC)

No unspecific amplification for NTC.

2. Positive Control

Assay is invalidated due to failed Positive control.

Well Position name type Sample Name CT Tm Melt Peak Height Results
H12 Positive POS Positive 25.83 82.61 202434.6 Positive Control Fail

IHC Controls

Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.

Well Position name type Sample Name CT Tm Melt Peak Height Results
A8 U01 IHC U01_IHC 24.87 85.04 242540.02 REVIEW: DPC Detected
C8 U02 IHC U02_IHC 24.86 84.90 257490.16 REVIEW: DPC Detected
E8 U03 IHC U03_IHC 24.90 84.90 245392.72 REVIEW: DPC Detected
E8 U03 IHC U03_IHC 24.90 77.27 98875.53 REVIEW: Failed IHC prevented call
G8 U04 IHC U04_IHC 24.82 84.90 239814.69 REVIEW: DPC Detected
G8 U04 IHC U04_IHC 24.82 77.27 117041.44 REVIEW: Failed IHC prevented call

4. Results

Samples that failed IHC are indicated in red.

CT for Unknown Samples

Well Position name type Sample Name CT Ct Mean Ct SD
A3 U01 UNK U01 34.28 35.13 1.25
A4 U01 UNK U01 34.54 35.13 1.25
A5 U01 UNK U01 36.56 35.13 1.25
C3 U02 UNK U02 32.23 32.71 0.49
C4 U02 UNK U02 32.69 32.71 0.49
C5 U02 UNK U02 33.21 32.71 0.49
E3 U03 UNK U03 35.32 35.57 0.45
E4 U03 UNK U03 36.09 35.57 0.45
E5 U03 UNK U03 35.31 35.57 0.45
G3 U04 UNK U04 32.50 32.89 0.41
G4 U04 UNK U04 32.87 32.89 0.41
G5 U04 UNK U04 33.31 32.89 0.41

Tm for Unknown Samples

Well Position name type Sample Name Tm Melt Peak Height
A3 U01 UNK U01 85.50 77278.47
A3 U01 UNK U01 75.36 123166.17
A3 U01 UNK U01 66.41 58898.02
A4 U01 UNK U01 75.49 120535.39
A4 U01 UNK U01 86.04 70206.27
A5 U01 UNK U01 75.39 104128.36
A5 U01 UNK U01 83.55 18516.14
A5 U01 UNK U01 62.28 49898.59
C3 U02 UNK U02 85.24 149437.91
C3 U02 UNK U02 74.56 90719.71
C4 U02 UNK U02 85.24 151773.92
C4 U02 UNK U02 77.63 140721.97
C5 U02 UNK U02 85.02 135553.25
C5 U02 UNK U02 75.12 98252.95
E3 U03 UNK U03 84.97 75935.55
E3 U03 UNK U03 75.09 65035.12
E3 U03 UNK U03 65.74 52871.05
E4 U03 UNK U03 74.96 131299.67
E4 U03 UNK U03 62.94 46572.74
E5 U03 UNK U03 74.98 85489.66
E5 U03 UNK U03 81.67 63229.24
G3 U04 UNK U04 85.10 141521.05
G3 U04 UNK U04 74.15 100195.68
G4 U04 UNK U04 74.82 139904.81
G4 U04 UNK U04 85.10 137059.50
G5 U04 UNK U04 76.86 121959.84
G5 U04 UNK U04 84.88 106743.47
End of Report