| Block Type | 96-Well 0.1-mL Block |
| Calibration Background is expired | No |
| Calibration Background performed on | 09-20-2022 |
| Calibration Pure Dye ABY is expired | No |
| Calibration Pure Dye ABY performed on | 09-20-2022 |
| Calibration Pure Dye CY5 is expired | No |
| Calibration Pure Dye CY5 performed on | 09-20-2022 |
| Calibration Pure Dye FAM is expired | No |
| Calibration Pure Dye FAM performed on | 09-20-2022 |
| Calibration Pure Dye JUN is expired | No |
| Calibration Pure Dye JUN performed on | 09-20-2022 |
| Calibration Pure Dye MUSTANG PURPLE is expired | No |
| Calibration Pure Dye MUSTANG PURPLE performed on | 09-20-2022 |
| Calibration Pure Dye NED is expired | No |
| Calibration Pure Dye NED performed on | 09-20-2022 |
| Calibration Pure Dye ROX is expired | No |
| Calibration Pure Dye ROX performed on | 09-20-2022 |
| Calibration Pure Dye SYBR is expired | No |
| Calibration Pure Dye SYBR performed on | 09-20-2022 |
| Calibration Pure Dye TAMRA is expired | No |
| Calibration Pure Dye TAMRA performed on | 09-20-2022 |
| Calibration Pure Dye VIC is expired | No |
| Calibration Pure Dye VIC performed on | 09-20-2022 |
| Calibration ROI is expired | No |
| Calibration ROI performed on | 09-20-2022 |
| Calibration Uniformity is expired | No |
| Calibration Uniformity performed on | 09-20-2022 |
| Chemistry | SYBR_GREEN |
| Date Created | 2022-12-22 09:06:36 AM SGT |
| Experiment Barcode | NA |
| Experiment Comment | NA |
| Experiment File Name | D:BiosystemsDesign & Analysis SoftwareFiles\2022-12-21_155355.eds |
| Experiment Name | 2022-12-21_155355 |
| Experiment Run End Time | 2022-12-21 17:41:38 PM SGT |
| Experiment Type | Standard Curve |
| Instrument Name | 272511079 |
| Instrument Serial Number | 272511079 |
| Instrument Type | QuantStudioâ„¢ 5 System |
| Passive Reference | ROX |
| Post-read Stage/Step | NA |
| Pre-read Stage/Step | NA |
| Quantification Cycle Method | Ct |
| Signal Smoothing On | true |
| Stage where Melt Analysis is performed | Stage3 |
| Stage/ Cycle where Ct Analysis is performed | Stage2, Step2 |
| User Name | Deborah Chen |
| Controls | Summary |
| Negative Control | Assay is invalidated see below for details. |
| Positive Control | Assay is invalidated due to failed Positive control. |
| Inhibition Control | Warning: These samples ( U01, U02, U03, U04 ) had failed IHC. |
No unspecific amplification for NTC.
Assay is invalidated due to failed Positive control.
| Well Position | name | type | Sample Name | CT | Tm | Melt Peak Height | Results |
|---|---|---|---|---|---|---|---|
| H12 | Positive | POS | Positive | 25.83 | 82.61 | 202434.6 | Positive Control Fail |
Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.
| Well Position | name | type | Sample Name | CT | Tm | Melt Peak Height | Results |
|---|---|---|---|---|---|---|---|
| A8 | U01 | IHC | U01_IHC | 24.87 | 85.04 | 242540.02 | REVIEW: DPC Detected |
| C8 | U02 | IHC | U02_IHC | 24.86 | 84.90 | 257490.16 | REVIEW: DPC Detected |
| E8 | U03 | IHC | U03_IHC | 24.90 | 84.90 | 245392.72 | REVIEW: DPC Detected |
| E8 | U03 | IHC | U03_IHC | 24.90 | 77.27 | 98875.53 | REVIEW: Failed IHC prevented call |
| G8 | U04 | IHC | U04_IHC | 24.82 | 84.90 | 239814.69 | REVIEW: DPC Detected |
| G8 | U04 | IHC | U04_IHC | 24.82 | 77.27 | 117041.44 | REVIEW: Failed IHC prevented call |
Samples that failed IHC are indicated in red.
| Well Position | name | type | Sample Name | CT | Ct Mean | Ct SD |
|---|---|---|---|---|---|---|
| A3 | U01 | UNK | U01 | 34.28 | 35.13 | 1.25 |
| A4 | U01 | UNK | U01 | 34.54 | 35.13 | 1.25 |
| A5 | U01 | UNK | U01 | 36.56 | 35.13 | 1.25 |
| C3 | U02 | UNK | U02 | 32.23 | 32.71 | 0.49 |
| C4 | U02 | UNK | U02 | 32.69 | 32.71 | 0.49 |
| C5 | U02 | UNK | U02 | 33.21 | 32.71 | 0.49 |
| E3 | U03 | UNK | U03 | 35.32 | 35.57 | 0.45 |
| E4 | U03 | UNK | U03 | 36.09 | 35.57 | 0.45 |
| E5 | U03 | UNK | U03 | 35.31 | 35.57 | 0.45 |
| G3 | U04 | UNK | U04 | 32.50 | 32.89 | 0.41 |
| G4 | U04 | UNK | U04 | 32.87 | 32.89 | 0.41 |
| G5 | U04 | UNK | U04 | 33.31 | 32.89 | 0.41 |
| Well Position | name | type | Sample Name | Tm | Melt Peak Height |
|---|---|---|---|---|---|
| A3 | U01 | UNK | U01 | 85.50 | 77278.47 |
| A3 | U01 | UNK | U01 | 75.36 | 123166.17 |
| A3 | U01 | UNK | U01 | 66.41 | 58898.02 |
| A4 | U01 | UNK | U01 | 75.49 | 120535.39 |
| A4 | U01 | UNK | U01 | 86.04 | 70206.27 |
| A5 | U01 | UNK | U01 | 75.39 | 104128.36 |
| A5 | U01 | UNK | U01 | 83.55 | 18516.14 |
| A5 | U01 | UNK | U01 | 62.28 | 49898.59 |
| C3 | U02 | UNK | U02 | 85.24 | 149437.91 |
| C3 | U02 | UNK | U02 | 74.56 | 90719.71 |
| C4 | U02 | UNK | U02 | 85.24 | 151773.92 |
| C4 | U02 | UNK | U02 | 77.63 | 140721.97 |
| C5 | U02 | UNK | U02 | 85.02 | 135553.25 |
| C5 | U02 | UNK | U02 | 75.12 | 98252.95 |
| E3 | U03 | UNK | U03 | 84.97 | 75935.55 |
| E3 | U03 | UNK | U03 | 75.09 | 65035.12 |
| E3 | U03 | UNK | U03 | 65.74 | 52871.05 |
| E4 | U03 | UNK | U03 | 74.96 | 131299.67 |
| E4 | U03 | UNK | U03 | 62.94 | 46572.74 |
| E5 | U03 | UNK | U03 | 74.98 | 85489.66 |
| E5 | U03 | UNK | U03 | 81.67 | 63229.24 |
| G3 | U04 | UNK | U04 | 85.10 | 141521.05 |
| G3 | U04 | UNK | U04 | 74.15 | 100195.68 |
| G4 | U04 | UNK | U04 | 74.82 | 139904.81 |
| G4 | U04 | UNK | U04 | 85.10 | 137059.50 |
| G5 | U04 | UNK | U04 | 76.86 | 121959.84 |
| G5 | U04 | UNK | U04 | 84.88 | 106743.47 |